Appearance of Xylella Fastidiosa in Pierce's Disease resistant and Susceptible Grapevines

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INHIBITION OF XYLELLA FASTIDIOSA POLYGALACTURONASE TO PRODUCE PIERCE’S DISEASE RESISTANT GRAPEVINES Project Leader:

Polygalacturonases (PG) (EC 3.2.1.15), catalyze the random hydrolysis of 1, 4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. Xylella fastidiosa (Xf) possesses a single PG gene, pglA (PD1485) and Xf mutants deficient in the production of PG result in lost pathogenicity and a compromised ability to systemically infect grapevines. We have cloned the pglA gene into a number o...

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Xylella fastidiosa requires polygalacturonase for colonization and pathogenicity in Vitis vinifera grapevines.

Xylella fastidiosa is the causal agent of Pierce's disease of grape, an economically significant disease for the grape industry. X. fastidiosa systemically colonizes the xylem elements of grapevines and is able to breach the pit pore membranes separating xylem vessels by unknown mechanisms. We hypothesized that X. fastidiosa utilizes cell wall degrading enzymes to break down pit membranes, base...

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THE ROLES THAT DIFFERENT PILI CLASSES IN XYLELLA FASTIDIOSA PLAY IN COLONIZATION OF GRAPEVINES AND PIERCE’S DISEASE PATHOGENESIS Project Leaders:

Type I and type IV pili of Xylella fastidiosa play different roles in twitching motility, biofilm formation, and cell-cell aggregation. Thirty twitching mutants were generated with an EZ::TN transposome system and type IV pilus-associated genes were identified, including fimT, pilX, pilY1, pilO, and pilR. Mutations in all resulted in a twitch-minus phenotype except that pilY1 mutant was twitchi...

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Identification of factors mediating cold therapy of Xylella fastidiosa-infected grapevines. PRINCIPAL INVESTIGATOR AND COOPERATOR

LIST OF OBJECTIVES AND DESCRIPTION OF ACTIVITIES CONDUCTED TO ACCOMPLISH EACH OBJECTIVE Objective 1: Develop an experimental, growth chamber temperature regime that can consistently cure Pierce’s Disease affected grapevines without causing unacceptable plant mortality. The results described in previous reports show that our field plants and cold chamber plants showed lower disease ratings and h...

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Transmission of Xylella fastidiosa to grapevines by Homalodisca coagulata (Hemiptera: Cicadellidae).

Pierce's disease (PD) of grapevines is caused by a xylem-limited bacterium Xylella fastidiosa (Wells, Raju, Hung, Weisburg, Mandelco-Paul, and Brenner) that is transmitted to plants by xylem sap-feeding insects. The introduction of the sharpshooter leafhopper Homalodisca coagulata (Say) into California has initiated new PD epidemics in southern California. In laboratory experiments, the major c...

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ژورنال

عنوان ژورنال: HortScience

سال: 2004

ISSN: 0018-5345,2327-9834

DOI: 10.21273/hortsci.39.4.826b